Overcoming the challenges of genetic instability in multicopy gene design

Abstract

In modern bioengineering, multicopy gene constructs are essential for reaching high protein yields and metabolic flux. However, these systems often suffer from severe genetic instability due to recombination and evolutionary pressure. Our team’s latest research, published in NAR Genomics and Bioinformatics, introduces ChimeraUGEM: a sophisticated tool that leverages biophysical modeling to design genetically stable multicopy sequences. By optimizing the "coding-regulatory" landscape, our tool allows researchers to maintain peak productivity throughout long-term fermentation without the typical loss of function. Dive into the methodology and see how we are redefining sequence stability.

Read More...