Default Expression Temp (°C)
E.coli                              30°C
Human                          32°C
Pichia Pastoris          25°C
Tobacco Plant            25°C
Chinese Hamster    32°C
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E.coli 
pET-21a(+) / Ampicillin
T7-based promoter with lac operator for E. coli expression; C-terminal 6 xHis tag (optional). IPTG induction with low copy number. 
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pET-24a(+) / Kanamycin
T7-based promoter with lac operator for E. coli expression; No affinity tags by default. IPTG induction with low copy number. 
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pET-28a(+) / Kanamycin
T7-based promoter with lac operator for E. coli expression; N-terminal 6 xHis/Thrombin site/T7 tag (optional) and C-terminal 6 xHis tag. IPTG induction with low copy number.
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pET-29b(+) / Kanamycin
T7-based promoter with lac operator for E. coli expression; N-terminal S-tag/Thrombin site and C-terminal 6 xHis tag. IPTG induction with low copy number.
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pET-11a (pET) / Ampicillin
T7-based promoter with lac operator for E. coli expression. No affinity tags. IPTG induction with low copy number.
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Human and CHO
pcDNA3.1(+) / Neomycin/G418
CMV-based promoter for mammalian expression; T7-based promoter 2 for in vitro transcription. High copy number with the AmpR gene for Ampicillin bacterial resistance. 

Pichia Pastoris
pPIC9
AOX1-based promoter for Komagataella phaffii expression. High copy number with the AmpR gene for Ampicillin bacterial resistance. Methanol induction with an AOX1, HIS4 recombination site and an α-factor secretion signal. 

pPIC9K
AOX1-based promoter for Komagataella phaffii expression. High copy number with the AmpR and KanR genes for Ampicillin and Kanamycin bacterial resistance, respectively. Methanol induction with an AOX1, HIS4 recombination site and an α-factor secretion signal. 
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pPICZα
AOX1-based promoter for Komagataella phaffii expression. High copy number with the BleoR gene for Zeocin bacterial resistance. Methanol induction with an AOX1 recombination site, C-terminal Myc/6xHis tag and α-factor secretion signal. 

pAO815
AOX1-based promoter for Komagataella phaffii expression. High copy number with the AmpR gene for Ampicillin bacterial resistance. Methanol induction with an AOX1, HIS4 recombination site. ​